The mobile section carries the sample elements throughout the column, in which they communicate with the stationary section to different levels. This conversation decides how long Each individual ingredient spends from the column, leading to their separation.
ディテクターから出力された、電気信号を記録し、そこからピークを検出、解釈を行う。結果は、感熱紙等に印字される。装置のコントロールをしないのであれば、どのメーカーの物を使用しても問題はないが、通常は、装置のコントロールも同時に行うため、同じメーカーの物を選択する。
試料を注入する部分で、手動式（マニュアルインジェクター）と自動式（オートインジェクター）がある。
Bear in mind, consulting your instrument guide and also the producer's specialized assist may also be precious resources when troubleshooting unique difficulties with all your HPLC system.
イオン交換クロマトグラフィーでは、無機イオンや高極性分子を電荷を利用して分離する。陽イオンタイプと陰イオンタイプの両方がある。イオン交換樹脂を利用する。
テキストはクリエイティブ・コモンズ 表示-継承ライセンスのもとで利用できます。追加の条件が適用される場合があります。詳細については利用規約を参照してください。
규제 약물(마약, 합성 마약, 대마, 각성제, 향정신성 의약품, 아편양제제 등), 반도핑 관련(금지 물질, 금지 약물, 스테로이드 등), 약물 대사물
As being a basic rule, a two unit modify during the polarity index corresponds to an approximately ten-fold transform inside a solute’s retention component. Below is a straightforward example. If a solute’s retention factor, k
Ghost peaks are extraneous peaks that look during the chromatogram but Really don't correspond to any factors inside the sample. These can complicate website facts Assessment. Here are some prospective triggers and answers:
Acid–base chemistry isn't the only illustration of a secondary equilibrium reaction. Other examples contain ion-pairing, complexation, along with the conversation of solutes with micelles. We are going to think about the past of these in Chapter twelve.7 whenever we go over micellar electrokinetic capillary chromatography.
The column would be the separation chamber wherever the magic of HPLC comes about. It properties the stationary stage, a packed bed of microscopic particles.
Lots of differing types of detectors happen to be use to watch HPLC separations, a lot of which use the spectroscopic tactics from Chapter ten or the electrochemical website procedures from Chapter 11.
To attenuate these issues we place a guard column prior to the analytical column. A Guard column usually has the identical particulate packing product and stationary phase given that the analytical column, but is drastically shorter and less expensive—a size of seven.5 mm and a value 1-tenth of that with the corresponding analytical column is regular. Since they are meant to be sacrificial, guard columns are replaced frequently.
An HPLC ordinarily consists of two columns: an analytical column, that's chargeable for the separation, along with a guard column which is positioned prior to the analytical column to protect it from contamination.